Search academic texts

Information × Registration Number 0218U000422, 0116U007116 , R & D reports Title Improving the maintenance and expansion of the collection of the National Center of microorganisms pathogenic for animals popup.stage_title Head Napnenko Oleksandr, Registration Date 29-01-2018 Organization The State Science-control institute of Biotechnology and microorganism's strains popup.description2 Report on research work is presented on 238 pages, contains 73 tables, 54 figures. The objects of the study were strains of microorganisms from the Collection of the National Center for the strains of microorganisms DNA and the isolates of Cronobacter spp. and Escherichia coli, bacteria of the genera Listeria and Pasteurella, pathogens of infectious diseases, test strain of CVS virus rabies, mumps; gene spaA strain "K" bacteria Erysipelothrix rhusiopathiae; 30 isolates of Salmonella enterica subsp. enterica; Integrated moderate phage in Salmonella enterica isolates and strains; genes of structural and non-structural proteins of Salmonella spp .; Methicillin-resistant (MRSA) isolates of Staphylococcus aureus; 12 strains of Bacillus anthracis; the B646L gene (VP72) of the African swine fever virus and the recombinant plasmid pUCB646L from the DNACRM collection; genes of structural and non-structural proteins of the viral diarrhea virus of cattle. The purpose of the work was to ensure epizootic well-being and to control the quality and safety of the VISA, disinfectants, antibacterial agents, and livestock food products. Methods of research - bacteriological, biochemical, physico-chemical, cultural, microscopic, biological, virological. The obtained results were processed statistically and mathematically using methods of variation statistics using the program "Microsoft Excel - 15,0" with the calculation of the arithmetic mean (M), the standard error (m) and the level of probability (p) for the Student table. The employees have been working on the selection of optimal conditions for conducting lyophilic drying of microorganisms and technologies for the production of nutrient media; the refreshing of microorganisms of the genus of listeria, pasterals, salmonella, clostridia and pathogenic viruses for animals was established and their compliance with passport characteristics was established; lyophilization and testing of the properties of test strains of microorganisms; new potentially sensitive cell cultures that can be used to test the cytotoxic activity of the compounds and isolate pathogen viruses; The Cronobacter spp-PCR-Test system for the detection of Enterobacter sakazakii (Cronobacter spp.) polymerase chain reaction (PCR) and a test system for the detection and identification of Schigatoxin producing E. coli variants was developed; Methodical recommendations for the determination of biological properties of bacteria of the genus Yersinia and thermophilic campylobacteria have been developed. In order to study the possibility of accumulation of bacterial mass for the production of biologics, existing and developed new systems for the cultivation of bacteria and viruses were studied. The polymerase chain reaction (PCR) method was used, different methods of DNA selection and purification, DNA sequencing, computer modeling of DNA and protein structure, phylogenetic heuristic analysis in two algorithms, DNA electrophoresis in agarose gel, microbiological methods. The main results of the research - the confirmed species conformance of 12 laboratory and vaccine strains of Bacillus anthracis; The specified nucleotide sequence of the spaA gene of the control unit is "K" Er. rhusiopathiae and phylogenetic analysis conducted; isolated and identified 7 isolates of S. aureus (MRSA); 50 local isolates of Salmonella enterica subsp. were investigated. enterica isolated in poultry farms of egg and meat type in Ukraine, and isolated isolates that are potentially highly pathogenic and antibiotic resistant; An integrated form of moderate phage has been found in 4 local isolates and 1 museum strain Salmonella enterica. Product Description popup.authors Акименко Л.І. Андрущенко В.В. Блоцька О. Ф. Войта О.С. Гаврасьєва Н.В. Гордієнко О. І. Дерябін О. М. Кісельова Т. Ф. Кацимон В.В. Клестова З.С. Компанієць І. В. Кудрявченко О.П. Кузьмич Г. С. Мандзя І.М. Напненко Олександр Олександрович Пінчук Н. Г. Поліщук І.В. Пустовіт Н. А. Романенко О. А. Рубленко Н.М. Салганська О.О. Семко К. Р. Тиндик В. С. Ющенко А.Ю. popup.nrat_date 2020-04-02 Close