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Information × Registration Number 0218U000921, 0117U003066 , R & D reports Title Development of effective biocatalysts: free and immobilized Agaricus bisporus tyrosinase for the screening of new inhibitors and the biosynthesis of L-3,4-dihydroxyphenylalanine popup.stage_title Head Romanovska Irina Igorivna, Доктор біологічних наук Registration Date 27-02-2018 Organization A.V. Bogatsky, Physico-Chemical Institute of the National Academy of Sciences of Ukraine popup.description2 Object of the investigation - tyrosinase inhibitors. From the Agaricus bisporus mushrooms, according to modified method, was isolated tyrosinase, with enhanced on 25 % activity. By the PAGE-SDS method the protein-fractional composition was determined (9 fraction); it was revealed, that 92,5 % the total protein demonstrates phenoloxidase activity. Screening of new tyrosinase inhibitors among the wide range of compounds: derivatives of 3-chloro-1?4-naphtoquinone, isatin, 3-hidroxy 2-naphthoic acid, etc., had shown, that significant influence on monophenolase activity of tyrosinase makes 2,7-dihydroxynaphtalene, benzylidenaniline and it's derivatives. It was established, that the most prospective inhibitor is 2-hydroxybensiliden-2-aminophenol, the IC50 of which is 3,5 mmol/dm3,what is 17-fold lower of that of classical inhibitor of melanogenesis - kojic acid. It was shown the belonging of benzylidenaniline to noncompetitive inhibitors of enzyme; values of inhibition constant variated in the wide range: from 5,1 to 254,6 mmol/dm3 , depending on the benzylidenanilines structure. Product Description popup.authors Декіна Світлана Сергіївна Романовська Ірина Ігорівна Севастьянов Олег Всеволодович Шестеренко Юлія Аркадіївна popup.nrat_date 2020-04-02 Close