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Information × Registration Number 0218U001065, 0115U002933 , R & D reports Title Creation of new biotechnological methods rhizobia strains resistant to unfavorable environmental conditions, to improve bacterial preparations popup.stage_title Head Kots Sergii Yaroslavovych, Registration Date 12-01-2018 Organization Institute of Plant Physiology and Genetics NASU popup.description2 As a result of the research it was established that Tn5 mutants of the strains B-16 and B-20 of B.japonicum obtained with using plasmid pSUP5011::Tn5mob as well as the strain T21-2 of B.japonicum that is Tn5 mutant obtained with using the plasmid pSUP2021::Tn5 produced low amount of exopolysaccharides and large number of protein under insufficient phosphor contents in the growing medium. Maximum number of protein was produced by the strain T21-2 of B.japonicum. While the new analytically selected strain AC88 of alfalfa nodule bacteria S.meliloti was cultivated in the conditions of phosphor deficit it produced large number of extracellular carbohydrate and low amount of protein, indicating the need for further research with the aim of using it as the component of the high effective bacterial preparations. As a result of screening of the Tn5 mutants (pSUP5011::Tn5mob) of nodule bacteria R.galegae, 12 bacterial cultures with improved symbiotic properties were selected. These bacteria can be used to form the effective symbiotic systems in galega plants. Product Description popup.authors Воробей Надія Анатоліївна ельникова Наталія Миколаївна Коць Сергій Ярославович Рибаченко Лілія Ігорівна popup.nrat_date 2020-04-02 Close