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Information × Registration Number 0218U001356, 0117U000532 , R & D reports Title To develop methodical approaches for predicting the stability of endangered race boars's sperm when using nanomaterials for its cryopreservation popup.stage_title Head Metlitska O.I., Registration Date 01-02-2018 Organization Institute of animals breeding and genetics UAAS popup.description2 The effectiveness of the cryopreservation method of ejaculated spermatozoa of Mirgorod breed boars of using nanomaterials was established.The method for determining the level of DNA fragmentation of decapperated boar spermatozoa depending on the concentration of nanomaterials in the composition of cryoprotectants has been optimized and practically applied.It was shown that the maximum activity after thawing is peculiar to spermatozoa, cryoconserved in the presence of HDC t200 ° C in 0.001% concentration - at 5.7% with survival for two hours.It was found that the number of spermatozoa from the fragmented DNA during cryopreservation with the addition of HDS t200 ° C did not exceed the limits of the physiological norm and amounted to 6.3%. The results of the obtained data formed the basis of the development of methodological recommendations for assessing the quality of sperm production of buds and the morphogenetic integrity of sperm as a component of work with small populations of animals of this species. Product Description popup.authors Ковтун С.І. Метлицька О.І. Палькіна М.Д. Стародуб Л.Ф. Цапко Л.О. Щербак О.В. popup.nrat_date 2020-04-02 Close