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Information × Registration Number 0218U001574, 0115U002920 , R & D reports Title Developing of biotechnology for accumulation of recombinant microRNA and proteins for needs of medicine. popup.stage_title Head Kuchuk Mykola Viktorovych, Registration Date 01-03-2018 Organization Institute of cell biology and genetic engineering NAS of Ukraine popup.description2 The selection of the most productive salad lines, which accumulate anti-PKC siRNA, is produced, the plant biomass grown in vitro is grown. The protocols for determining the level of accumulation of mature anti-PKC siRNA in transgenic plant salad lines have been developed. The conditions for the cultivation of Nicotiana benthamiana plants that are competent for transient agrobacterial transformation have been optimized for greenhouse growing. A stable genetic transformation of Nicotiana benthamiana plants using vectors that contain the alpha and beta subunits of human prolyl-4 hydroxylase genes has been carried out. Product Description popup.authors Василенко Максим Юрійович Сіндаровська Яна Рудольфівна popup.nrat_date 2020-04-02 Close