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Information × Registration Number 0218U002003, 0115U002950 , R & D reports Title Tumor suppressor genes and tumor-associated genes - as potential biomarkers for diagnosis and therapy of malignant tumors popup.stage_title Head Kashuba Vladimir Ivanovich, Доктор біологічних наук Registration Date 02-01-2018 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 The effect of C57Fb embryonic mouse fibroblasts (EMF) transduced with recombinant baculovirus (RB) encoding the murine Ifn-beta gene on the survival and proliferation of the B16 malignant mouse melanoma cells was studied on the model system.It has been shown that Ifn-beta, synthesized by C57Fb mouse embryonic fibroblasts or by B16 tumor cells, transduced with RB carrying the IFN-beta gene, inhibits the proliferation of B16 cells of mouse melanoma. The effectiveness of inhibition of the B16 cell proliferation was higher when the heterologous cells were co-cultured. Thus, the interaction between EMF and tumor cells mainly promotes the inhibition of tumor cell proliferation. We have shown that Ifn? is stably expressed for 120 hours in C57Fb cells transduced with RB Ac-M-Ifn?. It is noteworthy that the C57Fb / Ifn? cells were more resistant to the antiproliferative effect of Ifn? than B16 / Ifn-beta cells.5481 Product Description popup.authors Анопрієнко О.В. Вагіна І.М. Геращенко Г.В. Розенберг Є.Е. Чащина Л.І. popup.nrat_date 2020-04-02 Close