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Information × Registration Number 0218U002309, 0117U004099 , R & D reports Title Methodology of the forensic DNA-analysis popup.stage_title Head Abbasov Ruslan Hidaiaddynovych, Registration Date 14-02-2018 Organization THE STATE SCIENTIFIC RESEARCH FORENSIC CENTRE OF THE MINISTRY OF INTERNAL AFFAIRS OF UKRAINE popup.description2 From the beginning of the 80s of the XX century the technologies for the analysis of deoxyribonucleic acid (hereinafter - DNA) usage led to a revolution in molecular genetic studies. Human biological traces, which found at the crime scene, contain valuable information for the investigation. The discovering of a polymerase chain reaction (hereinafter - PCR) made it possible to increase the initial amount of biomaterial several times. It helped to identify a person using only a few picograms of human genomic DNA. For the first time, the composition of the ingredients included in the reaction mixture for performing PCR and the basic principles of using primers for obtaining copies of DNA was described by Clippe [Kleppe К. et al., 1971]. Cetus opened the basic principle of polymerase chain reaction - an exponential increase in the fragment of the original DNA number of copies in 1985 [Mullis K.B., 1990]. The usage of high-tech methods for analyzing DNA sequences with tandem repeats in their structure has become a breakthrough in forensic identification of a person. [Butler J.M., 2006]. A set of repeating sequences of a certain length is unique to each individual, except for identical twins. Depending on the size, repeats are divided on mini- and microsatellites [Pimenov M. и др., 2001]. For the first time the expediency of using hypervariable minisatellites (hereinafter VNTR locus) was reported by the prominent English geneticist Jeffreys in 1985 [Jeffreys A.J. et al., 1985a; 1985b]. During the 90s, microsatellites (hereinafter - "STR-locus") were widely used, which gave more opportunities for accurate identification of a person. [Fregeau C.J., Fourney R.M., 1993; Wang Y., 1995]. Since then, for more than 20 years, that STR-locuses are widely used in forensic DNA analysis. On their basis, the genomic information databases which help to solve countless cases in the criminal justice system were created. DNA is very stable molecule that can be stored for a long period of time. So, thanks to a large collection of DNA samples dating the early 90s, the US militaries were able to identify tens of thousands of dead soldiers [Butler J.M., 2010]. This methodology describes modern methods for human genetic features identification. Product Description popup.authors Повх А.С. Романчук С.М. popup.nrat_date 2020-04-02 Close