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Information × Registration Number 0218U002477, 0116U007099 , R & D reports Title Application of functional nanomaterials for creation of biosensor conductometric devices for arginine detection popup.stage_title Head Dzyadevych Sergei, Registration Date 21-03-2018 Organization Institute of molecular biology & genetics popup.description2 The method of research and selection of transducers for creation of a biosensor with optimal characteristics is proposed, due to forecasting of its maximum sensitivity and ensuring efficient operation in differential mode. Structures of measuring equipment, interfaces and measuring methods have been developed, structures and algorithms for work of bridge circuits with several working and reference conductivity transducers are offered, providing their automatic balancing with simultaneous diagnostics of the suitability of sensors. For the first time a conductometric microsensor based on clinoptilolite for selective determination of ammonium in complex aqueous media was developed. The possibility of conductometric determination of ammonium in the use of clinoptilolite without its preliminary preparation is shown. For the first time a conductivity biosensor was developed for the determination of L-arginine. The dependence of the biosensor reviews on the parameters of the buffer solution (pH, buffer capacity and ionic strength) and immobilization methods was studied. It has been shown that immobilization of arginase and urease by cross-linking with GA provides higher sensitivity of the biosensor to arginine and a wider linear measurement range (4.2 ?Sm / mM and 0.75-5 mM respectively) compared with the enzyme inclusion method in the polymer membrane (1.2 ?Sm / mM and 1-3 mM respectively). A biosensor manufactured in this way has demonstrated high operational stability and stability when stored (more than 4 months). For the first time clinoptilolite was used in a two-enzyme conductivity biosensor to determine L-arginine. It was shown that sorption and cation exchange properties of clinoptilolite, immobilized in the membrane based on arginase and urease, help to increase the sensitivity of the biosensor (from (11,205 ± 1,20)mkSm / mM to (19,025 ± 2,65) mkSm / mM). It was established that the preparation of a biosensor bioselective membrane by the method of adsorption of arginase and urease on zeolite with the subsequent application of the formed complex to the electrode surface, pre-zeolite modified, is the most effective way of manufacturing a highly sensitive biosensor for conductometric determination of L-arginine. It is suggested that ion exchange between sodium and ammonium, which is formed as a result of two stages of enzymatic cleavage of L-arginine, is an effective mechanism for ensuring a sufficiently high concentration of high-mobility charge carriers on the pre-electrode surface of the biosensor. Clinoptilolite containing enzyme biosensors for determination of L-arginine were characterized by high operational stability and stability during storage (more than 4 months). The developed biosensor has been successfully applied to determine L-arginine in samples of pharmaceutical products. The results are well correlated with the concentrations indicated by the manufacturers of these drugs. Product Description popup.authors Єльська Г.В. Архипова В.М. Дзядевич С.В. Зінченко О.А. Калініченко Л.А. Костюченко Н.І. Кучеренко І.С. Марченуо С.В. Мацишин М.Й. Пєшкова В.М. Пушкарьов В.О, Рачков О.Е. Сергеєва Т.А. Солдаткін О.О. Солдаткін О.П. Шкотова Л.В. popup.nrat_date 2020-04-02 Close