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Information × Registration Number 0218U003038, 0115U005044 , R & D reports Title Obtaining of genetically engineered immunoconjugates and their application for immunoaffinity chromatography and identification of target antigens popup.stage_title Head Gorbatiuk Oksana Borisivna, Кандидат біологічних наук Registration Date 12-01-2018 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 The genes of recombinant fusion proteins based on human interleukin-7 (rhIL7) with the C-terminal residue of oligohistydine (rhIL7-His) and the cellulose binding domain (rhIL7-CBD) were constructed. The optimal conditions for inducing the synthesis of recombinant proteins in Escherichia coli have been determined. The laboratory method for obtaining of rhIL7-His and rhIL7-CBD in a soluble form by renaturation was optimized. Immobilization of rhIL7-His was carried out on Ni-NTA sepharose and rhIL7-CBD on cellulose. The affinity mediums have been used for isolation the fraction of antibodies specific to rhIL7 from the serum of immunized animals. Product Description popup.authors Іродов Д.М. Похоленко Я.О. popup.nrat_date 2020-04-02 Close