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Information × Registration Number 0218U003090, 0115U004171 , R & D reports Title Investigation of mechanisms of plant stress adaptation and creation of tolerant lines using methods of experimental transgenesis popup.stage_title Head Shcherbak Nataliia Leonidivna, Кандидат біологічних наук Registration Date 17-01-2018 Organization Institute of cell biology and genetic engineering NAS of Ukraine popup.description2 The transgenic lines of potato and canola plants containing desaturase A gene cyanobacteria (desA) were selected using molecular biology analysis. A comparative analysis of the efficiency of expression of different types of constructions in different lines of transgenic plants was carried out. For the study of selected transgenic plants under stress conditions, methods of determination of a number of biochemical parameters determining the damage of plant cells were optimized and measurements, such as antioxidant system enzymes: superoxide dismutase (SOD), peroxidase, catalase; level of accumulation of malonic dialdehyde; level of proline release and the total antioxidant activity of the extracts. As a result of the studies, several lines of transgenic potato have been determined, which in vitro showed different from the control plants reaction on stress factors. Product Description popup.authors Кирпа-Несміян Тетяна Миколаївна Листван Катерина Володимирівна Овчаренко Ольга Олександрівна popup.nrat_date 2020-04-02 Close