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Information × Registration Number 0218U003640, 0113U002507 , R & D reports Title Development of bio / chemosensory analyzers to determine the content of formaldehyde, L- and D-lactate, their metrological assessment and experimental approbation. Development, metrological support and testing of formaldehyde analyzer on the basis of portable conductivity meter "MHR-3" and investigation of its adaptation to determine L- and D-lactate. popup.stage_title Head Korpan Yaroslav, Кандидат біологічних наук Registration Date 23-05-2018 Organization Institute of molecular biology & genetics popup.description2 The research was aimed at obtaining new bioselective elements of conductometric and impedimetric sensors for the determination of L-lactate. The analysis of several approaches to the formation of bioselective membranes on the surface of platinum thin-film planar electrodes with the use of biorecognition molecules of bacterial L-lactate dehydrogenase and pyruvate oxidase, in particular by cross-linking with glutaraldehyde and incorporation into polymeric films has been carried out. It has been established that both approaches are effective as they provide a good adhesion of the selective layer to the surface of platinum conductometric transducers. It was also found that the selective elements are highly sensitive (the detection limit for L-lactate is 25 ?M) and highly stable (the loss in signal value after 100 measurements is 5%). The main analytical characteristics of the created biosensor are investigated - dependence of signals on pH, buffer capacity, selectivity, specificity and stability during storage. The linear range for the detection of L-lactate concentration is in the range from 25 ?M to 1 mM, which makes possible the further use of such selective membranes for the analysis of biological fluids. Conductometric and impedimetric biosensors for determination of L-lactate were created. The optimization and testing of the sensors in model solutions and real samples (food products) was carried out. Product Description popup.authors Єльська Г.В. Архипова В.М. Білоіван О.А. Дзядевич С.В. Зінченко О.А. Калініченко Л.А. Корпан Я.І. Костюченко Н.І. Кукла О.Л. Кучеренко І.С. Марченко С.В. Мацишин М.Й. Пєшклва В.М. Пушкарьов В.М. Рачков О.Е. Сергеєва Т.А. Солдаткін О.О. Солдаткін О.П. Чабанова М.В. Шкотова Л.В. popup.nrat_date 2020-04-02 Close