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Information × Registration Number 0218U005115, 0116U004864 , R & D reports Title The application of metabolic engineering approaches for construction of Saccharomyces cerevisiae strains able to overproduction of glycerol under anaerobic conditions. popup.stage_title Head Dmytruk Kostyantyn Vasylovych, Registration Date 01-03-2018 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 To decrease the activities of the acetolacate syntase and alanine transaminase, recombinant strains of yeast Saccharomyces cerevisiae with the overexpression of truncated versions of ILV2 and ALT1 genes were obtained. Such shortened genes encode enzymes with cytoplasmic localization (while native enzymes have mitochondrial localization). The resulting recombinant strains produced, in different fermentation conditions, 1.5-4 times more glycerol than the wild type strain, but the combination of these modifications in the genome of one recombinant strain did not provide a further increase in the level of glycerol production. Overexpression of the ILV2 native gene encoding acetolacetate synthase with mitochondrial localization did not provide enhancement of glycerol production in S. cerevisiae. Product Description popup.authors Дмитрук Костянтин Васильович Куриленко Олена Олександрівна Семків Марта Віталіївна Сибірний Андрій Андрійович popup.nrat_date 2020-04-02 Close