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Information × Registration Number 0218U005578, 0116U003071 , R & D reports Title Explore the binary composition of Bradyrhizobium japonicum strains with different survival strategy and the possibility of its use as the basis of bacterial preparations popup.stage_title Head Krutylo Dmitry Valeryevich, Кандидат біологічних наук Registration Date 27-12-2018 Organization Institute of agricultural microbiology and agro-industrial production of National academy of agrarian sciences of Ukraine popup.description2 Object of research - nodule bacteria, binary composition of strains, soybean. The work purpose - to investigate the serological and genetic diversity of soybean nodule bacteria in the agrocenoses of Ukraine, to study the effectiveness of use the binary composition of B. japonicum strains as the basis of microbial preparations for soybean. Methods of researches: microbiological, molecular-genetic, serological, vegetative and field experiments, statistical. It was established that the heterogeneous populations of soybean rhizobia were formed in soils of different Ukraine regions. They representatives belong to 7 known serogroups: KB11, 46, M8, 634b, HR, OR, NR. Unidentified nodule bacteria are also found. Dominant in agrocenoses are intensive-growing rhizobia of KB11 serogroup. Significant genetic polymorphism of soybean nodule bacteria strains with slow and intensive growth was detected using the PCR-RFLP method of ITS-region. Based on 16S-23S rDNA restriction profiles, they are assigned to different ITS types. Restriction analysis of the rpoB gene revealed significant differences between soybean rhizobia strains with different growth rates. Using restriction enzymes MspI, HaeIII and NdeII, the intensive-growing strains are combined into one genetic group, while the slow-growing strains are assigned to two or three genetic groups. It was established that concomitant use of B. japonicum 46 and B. japonicum KB11 strains in a 1:1 ratio (binary composition) allows an increase the level of molecular nitrogen fixation and the above-ground mass of soybean plants of different varieties by 7.8-19.6% compared to monoinoculation. The positive influence on formation and functioning of symbiotic systems, the growth and development of soybean plants is caused not only strain-inoculants, but also their metabolism products. The ratio of local and introduced rhizobia in nodules changes significantly when strains with different growth rates and their filtrates used. Against the background of various monostrains rhizobia populations (B. japonicum KB11 and 634b), inoculation of soybean seeds by binary compositions of B. japonicum strains provided increase the level of nitrogen fixation and productivity of soybean. The largest increments in the ground mass of plant compared with the control (by 8.8-25.2%) were obtained by combining the strains of B. japonicum 46 and B. japonicum KB11. At the use of the created binary composition B. japonicum 46 + KB11 (Pat. N 114981, Ukraine) as the basis of Ryzobofit and Ryzohumin, there is no significant dominance of individual strains in nodules, they are in a state of dynamic equilibrium, and the formed symbiotic systems is balanced. The stable increases of soybean productivity are got as compared to control by 20-33%. Most effective is application of peat form of Ryzohumin. Scope of application - agriculture, the microbiological industry. Product Description popup.authors Крутило Д.В. Ушакова М.А. Шаховніна О.О. popup.nrat_date 2020-04-02 Close