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Information × Registration Number 0218U007140, 0118U001481 , R & D reports Title Engineering of xylose transport in the thermotolerant yeast Ogataea polymorpha for improvement of alcoholic fermentation popup.stage_title Head Sibirny Andriy Andriyovych, Registration Date 19-12-2018 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 Research object: the pathway of xylose conversion to ethanol in the thermotolerant yeast Ogataea polymorpha during alcoholic fermentation. The aim: metabolic engineering for improvement of the yeast Ogataea polymorpha for increased ethanol production during alcoholic fermentation. The aim of stage: to increase the efficiency of xylose transport by engineering of modified forms of O. polymorpha Hxt1 transporter. Methods: genetic engineering, microbiological, biochemical. Using genetic engineering approaches, O. polymorpha yeast strains were constructed with increased affinity of the Hxt1 transporter to xylose and reduced to glucose to achieve effective transport of both sugars into the cell. To increase the specific xylose uptake rate the modified Hxt1 was engineered by substitution of asparagine to alanine at position 358. Furthermore, N-terminal lysine residues of Hxt1 predicted to be the target of ubiquitination were replaced for arginine residues. The modified versions of Hxt1 was overexpressed in hxt1 mutant, unable to produce ethanol during high-temperature alcoholic fermentation. The efficiency of xylose and glucose co-utilization during high-temperature xylose fermentation was studied.5481 Product Description popup.authors Василишин Роксолана Василівна Куриленко Олена Олександрівна popup.nrat_date 2020-04-02 Close