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Information × Registration Number 0218U007778, 0116U001002 , R & D reports Title The research of the cryosensitivity and the factors of the resistance reduction of the umbilical cord blood cellular components to the freezing factors at the temperature of the liquid nitrogen. popup.stage_title Head Kalynychenko T.O., Доктор медичних наук Registration Date 18-12-2018 Organization Kiev Research Institute of Haematolojy and Blood Transfusion popup.description2 Research object: cord blood nuclear cells, cord blood red cell concentrate. Methods: сryobiological, morphological, biochemical, cultural, immunohematological. It has been demonstrated that the level of human umbilical cord blood (UCB) hemopoietic tissue (HT) cryosensitivity (due to procentage granulocyte-macrophage progenitors (GMPs) loss) has direct correlation with lipid peroxidation products (LPO) activity indices (with moderate and strong connection). The relative risk of loss of GMPs is significantly increased under the condition of high levels of LPO in UCB before beginning of the processing. Evaluation of the activity level of prooxidant processes in UCB using the biochemical markers before the start of the freezing procedure is valuable because of the possibility of creating an early prediction of the HT cryosensitivity, which can be useful in choosing cryopreservation tactics. It has been shown that antioxidants with different mechanisms of action (vitamin B1, B6 and B12 complex; ?-lipoid acid, thiazotic acid morpholinium salt, 2-ethyl-6-methyl-3-hydroxypyridine succinate) almost equally affect the oxidant status and equally increase stability of the umbilical cord blood nuclear cell suspensions to the cryopreservation negative factors. Medical and social effect. Medicine. Product Description popup.authors Аношина М.Ю. Балан В.В. Калиниченко Т.О. Мінченко Ж.М. Настенко О.П. Павлюк Р.П. Парубець Л.І. Тимошенко У.В. Шороп Є.В. Яговдік М.В. popup.nrat_date 2020-04-02 Close