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Information × Registration Number 0218U007912, 0117U006180 , R & D reports Title Investigation of response of plant cytoskeleton to the action of abiotic and biotic stress factors and improvement of their adaptive properties popup.stage_title Head Krasylenko Yu.A., Кандидат біологічних наук Registration Date 26-12-2018 Organization Institute of Food Biotechnology and Cenomics of the National Academy of Sciences of Ukraine popup.description2 The object of the study is the roots of four-day sprouts of A. thaliana (ecotype Columbia-0), roots and shoots of parasite plants of the Cuscuta europaea L., C. monogyna Vahl. The purpose of the study is to show the dependence of growth processes and morphogenesis of the main roots of seedlings of Arabidopsis thaliana and, respectively, the organization of microtubules and actin filaments on the influence of metals-pollutants (vanadium, chromium and lead) and elevated temperatures. Investigate the organization of actin and microtubule cytoskeleton of plant cells in parasite plants, for example, Cuscuta europaea L., C. monogyna Vahl. Methods of research - anatomical, cytological, physiological, methods of scientific research and statistical analysis. In the course of research it has been shown that abiotic factors, in particular ions of common soil pollutants such as lead, chromium and vanadium, inhibit the growth of major roots and cause morphogenesis of the roots. The effect of high temperatures (+ 38 ° C, + 41 ° C, + 45 ° C) also manifested itself in suppressing the growth of the major roots of Arabidopsis thaliana and is accompanied by changes in their morphology. We first visualized cytoskeleton in various organs (stems and rootstocks) in the early stages of ontogenesis (on the 1st to 7th day after germination) of common C. europaea L. and one-column (C. monogyna Vahl) with the help of immunohistochemical methods .) For the additional pharmacological confirmation of the presence of the components of parasite plants, the substances influencing the polymerization and the structure of the tubulin and actin: taxol, orrisalin, latrunulin B, cytohalazine B and D, jasplaninolide and BDM, which slowed germination of the seeds, inhibited the growth of seedlings and induced isodiative increase the size of the blubber cells. Product Description popup.authors Горюнова І.І. Плоховська С.Г. popup.nrat_date 2020-04-02 Close