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Information × Registration Number 0218U100091, 0116U008074 , R & D reports Title Polymorphism of DNA markers of artificial paddlefish (Polyodon spathula) populations popup.stage_title Head Ushkalov Valeriy , Доктор ветеринарних наук Registration Date 27-12-2018 Organization National University of Life and Environmental Sciences of Ukraine popup.description2 The object of the study is the polymorphism of DNA markers of artificial paddlefish populations. The aim of the study is to investigate the peculiarities of the genetic structure of artificial paddlefish populations using microsatellite DNA markers. The study used the following methods: molecular-genetic (nucleic acid exctraction, spectrophotometric determination of DNA concentration, amplification of DNA fragments by PCR method, detection of PCR-amplification products by horizontal electrophoresis, fragment analysis and genotyping of paddlefish by capillary electrophoresis); statistical (processing of experimental data using modern software packages). The theoretical and methodological basis of the research is the fundamental work of leading foreign and domestic researchers in the field of breeding, genetics of fish and aquaculture. The value of these results is to formulate recommendations for the practical application for fish farms for the implementation of molecular genetic identification and certification of the paddlefish brood stock. In order to manage the genetic resources of this species, farms need to apply an individual mark (chipping) to assign an individual genetic passport to each individual and create the genetic database. These results will allow to control over the gene pool conservation, formation of spawning pairs, reduce the level of inbred depression and establish the origin of both spawner individuals and their offspring and the paddlefish populations in general. Implementation of DNA identification and certification of brood stock in the practice of aquaculture at the state level will ensure control over the production, export and import of valuable fish products and will allow establishing and preventing the facts of falsification of this product on the market. The use of these approaches are possible in the field of aquaculture for management of genetic resources of artificially cultivated objects, for fish breeding farms with the the Stat Product Description popup.authors popup.nrat_date 2020-04-02 Close