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Information × Registration Number 0219U000001, 0115U002933 , R & D reports Title Creation of new biotechnological methods rhizobia strains resistant to unfavorable environmental conditions, to improve bacterial preparations popup.stage_title Head Kots Sergii Yaroslavovych, Registration Date 03-01-2019 Organization Institute of Plant Physiology and Genetics NASU popup.description2 It was shown that the strains of soybean nodule bacteria Bradyrhizobium japonicum B16 (Tn5 mutant with plasmid vector pSUP5011 :: Tn5mob) and T21-2 (Tn5 mutant with plasmid pSUP2021 :: Tn5) produced lower exopolysaccharides under addition into growth medium 0,5 g/l ammonium sulphate than 0,25 g/l. of above mentioned mineral compound. Mineral nitrogen (0,5 g/l) added inti culture medium stimulated protein biosynthesis by rhizobia S. meliloti AC88 and Tn5-mutants of soybean nodule bacteria. The growth media, such as yeast extract mannitol medium, sunthetic medium 79, pea medium, can be used to increase the biomass of nodule bacteria Rhizobium galegae (analytically selected and Tn5-mutants). This way will promote the increase of effectivity of bacterial composition for agriculture. Application of pea medium allows to obtain the culture of bacteria Rhizobium galegae, which are able to produce large amount of exopolysaccharides. Product Description popup.authors Воробей Надія Анатоліївна Коць Сергій Ярославович Мельникова Наталія Миколаївна Рибаченко Лілія Ігорівна popup.nrat_date 2020-04-02 Close