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Information × Registration Number 0219U000157, 0114U003217 , R & D reports Title The study of the formation mechanism of the fibrin framework of thrombus and the diagnostics tests development of the hemostasis system state at inflammation processes, blood vessels diseases and surgery. popup.stage_title Head Lugovskoi Eduard Vitaliiovich, Registration Date 17-01-2019 Organization A.V.Palladin Institute of Biochemistry of National Academy of Sciences popup.description2 Monoclonal antibodies I-5A were obtained. The epitope of its is situated in the fragment Aalpha 414-610 of the alpha C-domain of fibrinogen. Together with their Fab fragment antibodies I-5A inhibit the lateral association of protofibrils. Three short peptides Aalpha-91-103, Bbeta-125-135 and gama-69-77 were synthesized to mimic the aminoacid sequence of fragments of the hinge portion of the superspiral region of the fibrin molecule and inhibiting only the second stage of fibrin polymerization - the lateral association of protofibrils. The BbetaN domain (BbetaN 15-42) of fibrinogen was obtained, and it was found that the complex of the BbetaN domain and alphaC-region of fibrinogen is involved in the first stage of fibrin polymerization - the self-assembly of protofibrils. A library of fibrinogen-specific proteinases of animal and bacterial origin has been formed. Screened low molecular weight compounds - cyclooxygenase inhibitors according to their ability to inhibit ADP-induced platelet aggregation in platelet-rich human blood plasma. Based on monoclonal antibodies obtained using the synthesized peptides and the recombinant fragment of the protein C molecule, a preliminary version of the method for quantitative determination of protein C in human blood plasma was developed for diagnosing the threat of thrombus formation. The state of the hemostatic system of patients with chronic kidney disease (after hemodialysis) was described. A correlation was found between the parameters of the hemostatic potential, the concentration of molecular markers of thrombophilia and fibrinolysis, as well as a significant decrease in the activity of protein C. Product Description popup.authors Є.М. Макогоненко Є.М. Стогній І.М. Колєснікова А.В. Петік А.С. Дубовецький В.Є. Хаджинова В.І. Грищук В.І. Чернишов В.А. Соколовський В.В. Бутенко В.О. Наврулін Г.І. Горідько Г.К. Березницький Г.К. Гоголінська Д.С. Корольова М.О. Пидюра Н.Е. Луговська О.В. Горницька О.М. Губар О.Ю. Сломінський С.І. Андріанов Т.І. Курченко Т.В. Горошнікова Т.М. Платонова Т.М. Чернишенко popup.nrat_date 2020-04-02 Close