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Information × Registration Number 0219U000515, 0115U005201 , R & D reports Title Obtaining of cell lnes of agricultural plants with increased resistance to pathogenic and parasitic organisms by the way of inducing of RNA interference process using microbial bioregulators popup.stage_title Head Spivak Svitlana Igorivna, Кандидат біологічних наук Registration Date 04-02-2019 Organization Institute of Food Biotechnology and Cenomics of the National Academy of Sciences of Ukraine popup.description2 The morpho-physiological indices of resistance to parasitic nematodes of obtained in vitro onnutrient mediacontaining bioregulators of microbiological origin of tomato plant-regenerants(Lycopersicon esculentum Mill.)cultivar Lagidny were studied in greenhouse conditions.It was shown that under greenhouse conditions, the resistance to nematode invasion of tomato plants-regenerants obtained in vitro on nutrient media containing bioregulators of microbiological origin, used in concentrations: 50, 75 and 100 ?l/l, significantly increases(up to 26-99%).A significant differences in the percentage of hybridized mRNA and si/miRNA molecules (up to 40 - 69 %) isolated from cells of control and experimental tomato plants-regenerants, obtained in vitro on nutrient media containing bioregulators of microbiological origin and further grown in greenhouse conditions on natural invasive background,were set.In the cell-free system of protein synthesis from wheat seedlings it was shown the significant increase in the silencing (inhibiting translation of plant mRNA) activity of si/miRNA (up to 36 - 66%) isolated from cells of experimental tomato plants-regenerants, obtained in vitro on nutrient media with bioregulators of microbiological originand further grown in greenhouse conditions on natural invasive background, as compared with similar indices of control tomatoplants-regenerants. Product Description popup.authors Іутинська Г.О. Білявська Н.О. Співак С.І. Циганкова В.А. Шиша О.М. popup.nrat_date 2020-04-02 Close
R & D report
Obtaining of cell lnes of agricultural plants with increased resistance to pathogenic and parasitic organisms by the way of inducing of RNA interference process using microbial bioregulators
Head: Spivak Svitlana Igorivna. Obtaining of cell lnes of agricultural plants with increased resistance to pathogenic and parasitic organisms by the way of inducing of RNA interference process using microbial bioregulators. (popup.stage: ). Institute of Food Biotechnology and Cenomics of the National Academy of Sciences of Ukraine. № 0219U000515
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Updated: 2026-03-25


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