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Information × Registration Number 0219U000536, 0115U005044 , R & D reports Title Obtaining of genetically engineered immunoconjugates and their application for immunoaffinity chromatography and identification of target antigens popup.stage_title Head Gorbatiuk Oksana Borisivna, Кандидат біологічних наук Registration Date 06-02-2019 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 A recombinant fusion protein beta-Lac-SPA containing two functional domains - the Staphylococcus aureus protein A, which interacts with the Fc-parts of IgG and bacterial beta-lactamase immune complexes visualization was constructed. Conditions of induction of ?-Lac-SPA synthesis in Escherichia coli, which ensured its accumulation in a soluble form have been determined. The fusion protein ?-Lac-SPA, after isolation and purification, has been tested for the detection of immune complexes in different types of immunoassay and Western Blot. Since Staphylococcus aureus protein A interact with IgG of different species of animals and humans, fusion protein beta-Lac-SPA can be used as a universal secondary immunoassay tools. Product Description popup.authors Іродов Д.М. Похоленко Я.О. popup.nrat_date 2020-04-02 Close