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Information × Registration Number 0219U000871, 0115U004200 , R & D reports Title Modeling of molecular processes underlying Parkinson's disease in yeast cells and analyzing the impact of exogenous and endogenous factors on the processes of aggregation and degradation of human alpha-synuclein popup.stage_title Head Stasyk Oleh Volodymyrovych, Кандидат біологічних наук Registration Date 07-03-2019 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 Using the previously engineered H. polymorpha yeast strains, the effect of recombinant alpha-synuclein on the state of the prooxidant-antioxidant system and vitality of cells in the presence of excess Mn (II) and Cu (II) ions in the culture medium was investigated. During the research, it was found that with an increase of the concentrations of Mn (II) and Cu (II) ions in the medium, their toxic effect on the growth of strains was more pronounced in the wild strain than in the model strain in which recombinant human alpha-synuclein was synthesized. It can testify to the chelator properties of this protein. In addition, the excess of Mn (II) and Cu (II) ions led to a more pronounced increase in the content of lipid modification products and catalase activity in wild type strains of cell lysates compared to the alpha synuclein producing strain. The influence of alpha-synuclein on the oxidative modification of proteins was not very pronounced. Ions Mn (II) and Cu (II) had an inhibitory effect on the biosynthesis of the synuclein, but did not cause its aggregation. Product Description popup.authors Вовк Олена Іванівна Маєвська Оксана Михайлівна Стасик Олег Володимирович popup.nrat_date 2020-04-02 Close