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Information × Registration Number 0219U000891, 0118U005171 , R & D reports Title Affinity sensor system based on "smart" hybrid nanocomplexes for determination of specific nucleic acid sequences. Section 2. Development of "smart" hybrid nanocomplexes popup.stage_title Head Rachkov Oleksandr, Registration Date 13-03-2019 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 For the development of an affine sensory system based on "smart" hybrid nanocomplexes to detect the expression level of the hybrid gene bcr-abl, the protein product of which induces the development of Ph'-positive forms of leukemia, it was necessary to select the size and nucleotide sequence of at least three single-chain oligonucleotides (one relatively long and two relatively short). A long oligonucleotide serving as a target should reproduce the nucleotide sequence of the hybrid mRNA of the docking region of the corresponding exons of the original genes bcr and abl and have sufficient length so that the regions complementary to the two short oligonucleotides are not overlapped. 80-bcr-abl 80-mer oligonucleotide, 19-mer MP-19 oligonucleotide (complementary to the docking site), and the 18-mer oligonucleotide DP-18 (complementary to the 5'-terminal portion of the 80-bcr-abl oligonucleotide) correspond to such conditions. Product Description popup.authors Бахмачук Анна Олегівна Рачков Олександр Едуардович popup.nrat_date 2020-04-02 Close