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Information × Registration Number 0219U000899, 0117U003066 , R & D reports Title Development of effective biocatalysts: free and immobilized Agaricus bisporus tyrosinase for the screening of new inhibitors and the biosynthesis of L-3,4-dihydroxyphenylalanine popup.stage_title Head Romanovska Irina Igorivna, Доктор біологічних наук Registration Date 13-03-2019 Organization A.V. Bogatsky, Physico-Chemical Institute of the National Academy of Sciences of Ukraine popup.description2 From the Agaricus bisporus mushrooms, using modified method, was isolated tyrosinase with increased on 25% activity. The effect of 22 synthesized benzylideneananilines and related compounds on tyrosinase activity was studied, 7 of them are effective enzyme inhibitors. The molecular docking method shown embedding benzylideneanilines in the catalytic pocket of tyrosinase. The most important structural parameter is the presence of an OH-group at the 2-position of the aminophenol ring. QSPR analysis of the relationship structure of benzylidenanilines - the effectiveness of tyrosinase inhibition showed, that lipophilicity is the most significant factor (~ 73.8%). The synthesis of L-DOPA was carried out using isolated tyrosinase in methylene chloride, the formation of the product was 30%. Product Description popup.authors Романовська Ірина Ігорівна Севастьянов Олег Всеволодович Шестеренко Євгенія Аркадіївна Шестеренко Юлія Аркадіївна popup.nrat_date 2020-04-02 Close