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Information × Registration Number 0219U001209, 0115U001362 , R & D reports Title Genetic control of riboflavin biosynthesis and transport in flavinogenic yeasts popup.stage_title Head Fedorovych Dariya Vasylivna, Registration Date 18-01-2019 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 The strains of Candida famata yeast with overexpressed genes PRS3 (encoding phosphoribosyl pyrophosphate synthetase) and ADE4 (encoding phosphorybosylpirofosfamidotransferase) involved in the biosynthesis of the predecessor riboflavin - GTP de novo, were constructed . It has been shown that in a strain that contains, in addition to the overexpressed genes of the transcription factor SEF1 and the structural genes of the biosynthesis of riboflavin RIB1 and RIB7, a plasmid with both modified PRS3 and ADE4 genes, the riboflavin production increased in 2.5-3.5 fold . Riboflavin production reached 365 mg / ml in Berkholder's medium, 1168 mg / ml in Berkholder's medium supplemented with yeast extract, and 884 mg / ml in YPD medium.The C. famata strains with an overexpressed BCRP (breast cancer homologue) gene encoding the system of riboflavin excretion of the genetically similar species of the C. famata yeast, Debaryomyces hansenii, were constructed/ These strains were characterized by 1.3 to 1.5-fold increase in riboflavin production compared with a recipient strain containing two copies of the genes SEF1, RIB1 and RIB7. Product Description popup.authors Дмитрук Костянтин Васильович Сибірний Андрій Андрійович Фаюра Любов Романівна Цирульник Андрій Олександрович popup.nrat_date 2020-04-02 Close