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Information × Registration Number 0219U001210, 0116U002209 , R & D reports Title Genetic and biochemical aspects of the regulation of some catabolic and anabolic processes in microorganisms: alcoholic fermentation, catabolism of methanol, biosynthesis of flavins, glycerol, hydrogen and glutathione popup.stage_title Head Sibirny Andriy Andrijovych, Registration Date 18-01-2019 Organization Institute of cell biology, National Academy of Sciences of Ukraine popup.description2 Strains with a weakened expression of the TPI1 gene encoding the triazophosphatizomerase were constructed; Expression of genes GPD1 (encoding glycerol-3-phosphate dehydrogenase), GPP2 (encoding glycerol-3-phosphate dephosphatase), fusion gene of GPD1-GPP2fus, and a shortened ILV2trunc gene (encoding the cytoplasmic form of the acetylacetate synthase enzyme) under the control of a strong constitutive ADH1 promoter were obtained. In order to increase the export of glycerin from S. cerevisiae cells to the environment, a vector for the expression of a shortened form of the FPS1 gene (encoding a modified membrane transporter of glycerol) was introduced into the S. cerevisiae gene under the control of the ADH1 promoter . Subsequently, all of these mutations weree combined in the genome of one recombinant S. cerevisiae strain. The resulting best producer synthesized 9 times more glycerol in microaerobic conditions and 4.7 times more glycerol in anaerobic conditions when compared with a wild strain BY4742. Product Description popup.authors Андреєва Ю. Борбуляк М.І., Братійчук Д.І. Булботка Н. Василишин Р. Дмитрук К.В. Дмитрук О.В. Зозуля А.З Колодій О.М. Куриленко О.О. Павлюх Х.В Петровська Я. Семків М.В. Фаюра Л.Р. Федоренко В.О. Федорович Д.В. Цирульник А.О. Шевчук Н. В Юрків М. popup.nrat_date 2020-04-02 Close