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Information × Registration Number 0219U001303, 0117U004312 , R & D reports Title To investigate of genetic mechanism transformation of abnormal karyotypes in B-cells chronic lymphocytic leukemia and multiple myeloma popup.stage_title Head Andreieva Svitlana V., Registration Date 23-01-2019 Organization State Institution "Institute of Haematology and Transfusiology of NAMS of Ukraine" popup.description2 Object: interphase nuclei and metaphase chromosomes of bone marrow (BM) cells and B-blasttransformed lymphocytes of peripheral blood at relapse of B-cells chronic lymphocytic leukemia (B-CLL) (61 patients) and multiple myeloma (ММ) (62 patients). Methods of investigation: karyotyping, i-FISH, clinical, haematological, morphologycal. Aim: to estimate spectrum of quantity and structural chromomal abnormalities in bone marrow cells at relapse of B-CLL and MM . Results: by karyotyping more often were revealed sn relapse of B-CLL (48,2 %), diploidy clones - in MM (81,3 %)..Tthe deletions are predominated between structutal rearrangements (63,8 %), and chromosomes17, 13 and 11 (25,5 %. 16,4 % та 12,7 %, karyotyping), genes ATM and TP53 (i-FISH analysis) in B-CLL. Comparison results of karyotyping of BM cells and nd B-blasttransformed lymphocytes of peripheral blood was revealed different chromosomal abnormalities, which evidence about different origin. In relapse of ММ in structural rearrangements were often involved chromosomes 1, 7 and 19. Between structural abnormalities were dominated balanced translocations and derivatives chromosomes. Unfavourable prognosis were registered together karyotyping and i-FISH method (relapse of В-CLL and ММ - 66,7 % и 32,6 %,68,4 %, respectively). Atypical distributon of signals in MM were formed by increased quantities signals of locus 4p16, 6p21, 11q13, 14q32, 16q23 and decreased intensivities of locus 6p21, 16q23, that can evidenced about new mechanisms to form abnormal clones. Medical-social effect. Medicine. Product Description popup.authors Андреєва Світлана Василівна Гордієнко Алла Іванівна Корець Катерина Веніамінівна Кубарова Валентина Олександрівна Перехрестенко Тетяна Петрівна Сівкович Світлана Олексіївна popup.nrat_date 2020-04-02 Close