Search academic texts

Information × Registration Number 0219U001395, 0116U000140 , R & D reports Title Study of regenerative potential of endothelial progenitor cells at ischemic tissue injuries. popup.stage_title Head Gennadii M. Butenko, Registration Date 28-01-2019 Organization Institute of genetic and regenerative medicine Acad.Med.Sci. of Ukraine popup.description2 The protocols for isolating and culturing progenitor cells from the murine aorta were developed, as well as their morphological and phenotypic characteristics in the dynamics of culture were described. The use of Matrigel matrix has been found to promote the proliferation of aorta-derived progenitors and expression of endothelial markers. Protocols for multiparametric immunophenotyping of endothelial progenitor cells by flow cytometry using CD31, CD38, CD34, CD45 and CD309 monoclonal antibodies were developed. On the model of critical limb ischemia in mice, the regenerative potential of endothelial progenitor cells from the murine aorta was established, which was manifested in the restoration of the perfusion of ischemic tissues and the improvement of histological parameters of muscle tissue. The in vitro model of periventricular leukomalacia demonstrated that adipose-derived MMSC at non-contact co-culture with mouse brain slices has a neuroprotective effect on nervous tissue, reducing reactive astro- and microgliosis and increasing the number of Rip+ oligodendrocytes. It has been demonstrated that co-culture with organotypic brain slices of the adipose-derived MMSC improves the cytoarchitecture of the ischemic hippocampus, which are able to integrate into its tissue and can express the neuronal marker NeuN. It has been shown that neural progenitor cells transplanted stereotactically in ischemic hippocampus of mice are able to stimulate endogenous neurogenesis in the subgranular zone of the dentate gyrus and thus contribute to the recovery of lost functions. At the same time, neural progenitor cells transformed for overexpression of extracellular matrix metalloproteinases inductor EMMPRIN are able to migrate from the subventricular zone of the lateral ventricle to the damaged somatosensory cortex further than the control CPD and can form a population of neural progenitors in the damaged cortex. An experimental models of isoproterenol-induced ischemic cardiomyopathy and combined ischemic-autoimmune neonatal periventricular leukomalacia in vitro, as well as protocols for magnetic sorting subpopulations of progenitor cells from mouse brain, which can be used for further studies of the regenerative capacity of stem and progenitor cells of various types. Product Description popup.authors Кирик Віталій Михайлович Клименко Павло Павлович Кондратенко Костянтин Миколайович Луценко Тамара Миколаївна Рибачук Оксана Андріївна Скибо Галина Григорівна Устименко Аліна Миколаївна Цупиков Олег Михайлович Шматко Галина Андріївна popup.nrat_date 2020-04-02 Close