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Information × Registration Number 0219U001799, 0118U005178 , R & D reports Title Development, optimization and testing of printed ampereometric epidermal sensing system for the non-invasive detection of L-lactate. popup.stage_title Head Korpan Yaroslav Izidorovich, Кандидат біологічних наук Registration Date 01-03-2019 Organization Institute of Molecular Biology and Genetics of NASU popup.description2 Object of research - nanocomposite sensors based on printed transducers to determine L-lactate.The purpose of the work - Development of effective ways to modify the surface of printed amperometric electrodes to determine L-lactate.Electrochemical analysis of L-lactate is provided by detecting the reduced nicotinamide adenine dinucleotide (NADH) as a product of enzyme transformation involving lactate dehydrogenase (LDH). The study involved the development of a high-performance nanocomposite electrode for monitoring NADN and optimization of L-lactate analysis in the presence of LDH and NAD +. The paper uses carbon screen-printed electrodes (SPE). The surface of the working carbon electrodes is modified by N-graphene (NG), detonating nano-diamonds (dND) and nanocomposite films with polymers. The influence of different modifications of SPE on their electrochemical properties and the effectiveness of NADN electrooxidation by cyclic voltammetry, electrochemical impedance spectroscopy and amperometry are investigated. Optimized electrodes are used to analyze L-lactate .The procedure is optimized and the principal possibility of L-lactate determination in the range 1-50 mM is shown with the help of sensors developed, which will be applied in the next stage of research to create the appropriate biosensors with the immobilized enzyme matrix. Product Description popup.authors Білоіван О.А. Жибак М.Т. popup.nrat_date 2020-04-02 Close