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0219U001831,
0115U004198
,
Improvement of gene delivery efficiency into plants by using novel nanoscale polycationic polymers
Stoika Rostyslav,
07-03-2019
Institute of cell biology, National Academy of Sciences of Ukraine
We have explored the possibility of application of polymers that were synthesized at the Department of Organic Chemistry of the National University "Lviv Polytechnic" for the delivery of foreign genetic material into plant cells. Polymer TN 83/6 at a concentration of 0.001% increased the transformation frequency of tomato Lycopersicon esculentum Mill. (Lahidnyi variety) from 6.8% to 13.3%, tomatoes of the Money Maker variety - from 13.4% to 23.8%, compared to the frequency of Agrobacterium-based transformation. This frequency also increased to 39.6% when a polymer TN 84/5 was used at a concentration of 0.001%. The cytotoxicity of the polymers for the barley Hordeum vulgare L. of the Achilles variety callus increases in a row: F8-DM3> TN 83/6> BArc-DM2> TN84/5> LArc-DM2> BArc-DM1> F8-DM4> LArc-DM1. The transient expression of YFP gene was detected under the aplication of all polymers for the transformation of barley callus, and was not observed in control samples (without the addition of a polymeric carriers). New polymers have also demonstrated the ability to deliver plasmid DNA in protoplasts of moss Ceratodon rurpureus and low toxicity towards moss protoplasts. The F8-DM-2 fluorinated polymeric carrier possessed the highest efficiency to deliver the plasmid DNA in the C. rurpureus moss protoplasts. Thus, the method of plant transformation using polymers based on DMAEM is promising for the transformation of various systematic groups of plants.
Ключівська Ольга Юріївна
Фінюк Наталія Степанівна
2020-04-02
Updated: 2025-12-08
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