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Information × Registration Number 0219U003072, 0116U001003 , R & D reports Title The investigation of cytogenetic effects peculiarities in radiotherapy patients due to different volume and regimen of irradiation popup.stage_title Head Maznyk N.O., Registration Date 17-01-2019 Organization State Organization "Grigoriev Institute for Medical Radiologiy of the National Academy of Medical Sciences of Ukraine" popup.description2 Object of study: cytogenetic effects in cultured peripheral blood lymphocytes of cancer patients during radiation therapy with various regimen and irradiation volumes. The aim: to assess the cytogenetic effects in vivo and ex vivo in lymphocytes of cancer patients following external and internal radiation treatment, depending on the irradiation volume and exposure regimen. Research methods and techniques: cytogenetic analysis of human blood cultured lymphocytes, statistical methods of data processing, microscopes Axioskop, Micros MC300X and Olympus, system ASIS. Theoretical and practical results: The peculiarities of chromosomal aberrations outcome and their distribution in human lymphocytes irradiated in therapeutic doses ex vivo with simulated local radiation exposure were shown. The level of chromosomal exchanges during ex vivo irradiation at 2 Gy was significantly higher than spontaneous and exceeded 20 % - 25 % in all patient groups. The highest level of chromosomal damage was found in patient group with lung cancer at a dose of 4 Gy. A high informativity of cytogenetic test in vivo and ex vivo and of selected experimental model for detecting local irradiation and radiosensitivity of lymphocytes in patients with different tumor localization were demonstrated. These data are of importance for correct assessing the impact of therapeutic irradiation on normal tissues at the chromosomal level. Novelty: new knowledge was obtained about the character of chromosome damage outcome due to ex vivo irradiation of lymphocytes in cancer patients with different tumor localization. The effectiveness of implementation: is not present. Area of application: radiobiology, radiation therapy, biological dosimetry, radiation medicine. Product Description popup.authors Артюх С.В. Білозор Н.В. Гайсенюк Л.О. Дашкова С.А. Колпакова Л.К. Кругова І. М. Мазник Н.О. Пшенічна Н.Д. Пшишевська Л.А. Святенко Н.І. Сипко Т.С. Старенький В.П. Сухіна О. М. popup.nrat_date 2020-04-02 Close