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Information × Registration Number 0219U003241, 0116U004778 , R & D reports Title Molecular typing and mechanisms of evolutionary adaptation of viruses of cultural and wild-growing flora of Ukraine popup.stage_title Head Budzanivska Irene Genadiivna, Registration Date 29-01-2019 Organization Taras Shevchenko Kiev University popup.description2 Research objects: viruses of cultural and wild-growing plants, phylogenetic properties, evolution, adaptation mechanisms, epidemiological factors, virus ecology. Aim of the work: data bank of genetic sequences of plant viruses from Ukraine, and improvement of test systems for their diagnostics. Research methods: methods of virology, molecular biology, phylogeny and statistics. We have collected isolates of harmful viruses infecting cultural and wild-growing plants in Ukraine, and studied their biological, structural and biochemical properties. 'Marker' genome sequences have been identified which resulted in the development of cDNA libraries of isolated viruses. Partial and full genomic sequences of certain viruses were sequenced and analyzed using bioinformatics approaches. Phylogenetic analysis of obtained amplicons and their comparison with published genetic sequences were conducted. Directions and biologically significant mechanisms of microevolutionary adaptation of Ukrainian isolates of plant viruses were established. Epidemic potential of Ukrainian isolates of plant viruses was evaluated and correllated with biotic or abiotic factors. We have developed and modified existing test systems for molecular diagnostics of certain plant viruses. Unique object-oriented data bank of genetic sequences of plant viruses isolated in Ukraine has been developed. Product Description popup.authors Бисов А.С. Будзанівська І.Г. Дуніч А.А. Міщенко Л.Т. Руднєва Т.О. Снігур Г.О. Шевченко О.В. Шевченко Т.П. popup.nrat_date 2020-04-02 Close