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Information × Registration Number 0219U003365, 0116U000266 , R & D reports Title Development of the modern diagnostic tools for animal brucellosis monitoring based on molecular genetic methods popup.stage_title Head Zavgorodniy A.I., Bolotin V.I., Registration Date 06-02-2019 Organization National Scientific center "Institute of experimental and clinical veterinary medicine" popup.description2 The aim of the work was to study the molecular genetic characteristics of Brucella of various species and to develop the SOP of the species identification of Brucella by PCR and a test system for the detection of B. ovis genetic material by real-time PCR. The SOP (laboratory regulations) for species identification of brucella (B. abortus, B. melitensis, B. suis, B. ovis) by PCR was developed. Based on the study of different genes (n = 71) of different B. ovis strains (IS711, 16S and 23S rRNA, Ykwd, HSP70, HSP40, CSP, Omp2, Omp31, ABC transporter, specific oligonucleotides were designed for real-time PCR. A diagnostic kit has been elaborated for the indication of B.ovis genetic material on real-time PCR. An instruction for the manufacture and control of this kit was prepared. Product Description popup.authors Болотін В.І. Калініченко Т.В. Куценко В.А. Марченко Н.В. Обуховська О.В. Орехова Г.А. Орлов С.М. Пікун О.Ю. Рамазанова Т.П. Солодянкін О.С. popup.nrat_date 2020-04-02 Close