Search academic texts

Information × Registration Number 0219U003458, 0116U001494 , R & D reports Title The intercellular communication system functioning in Pseudomonas aeruginosa strains with different levels of autoinducers and second messengers biosynthesis. popup.stage_title Head Filipova Tetiana Olegivna, Registration Date 28-02-2019 Organization Odesa I.I.Mechnikov national university.Pervomaisk institute. popup.description2 The possibility of increasing the biosynthesis of biosurfactants by using bacterial signaling molecules, in particular, P. aeruginosa PQS, has been experimentally proved and the role of the cyclo-di-GMP exchange system in this process has been determined. The role of the second messenger has been elucidated by using, in addition to the wild type strain P. aeruginosa PA01, its mutants with a low and elevated level of cyclo-di- GMP biosynthesis: P. aeruginosa PA01 pJN2133 and P. aeruginosa PA01 ? wspF1, respectively. The results of the research work allowed us to formulate the following concept: Increasing of biosynthesis of the rhinolipid, the main biosurfactant of P. aeruginosa, occurs against the background of an increased content of signaling quinolone and a decrease in the concentration of cyclo-di- GMP in the P. aeruginosa cells cytoplasm. The rationale for this concept is the data obtained for the first time on the higher level of biosynthesis of the rhamnolipids and the increased content of PQS in P. aeruginosa PA01 pJN2133. That is, a feedback is found between the level of the secondary messenger and the ability to produce biosurfactants. The obtained results are the basis for new approaches to enhancing the biosynthesis of various useful metabolites of bacteria, in particular, biosurfactants, by: 1. application of exogenous regulatory molecules that affect the level of biosynthesis of secondary messengers and the functioning of the Quorum Sensing system; 2. development and use of bioplastic reactors; 3. molecular design and directed synthesis of new substances capable of modifying the content of cyclo-di- GMP in producer cells; 4. Use of bacterial strains with a naturally low level of cyclo-di- GMP biosynthesis. Obtained data are also of practical value for developments in the medical field. They indicate the possibility of using substances that can reduce the level of cyclo-di-GMP, for the modification of materials from which catheters, tracheal tubes, implants, etc. are made, in order to prevent the formation of biofilms.It was shown that PQS and its synthetic derivatives with different alkyl chain length modulate biofilm formation process in P. aeruginosа depends of intracellular level of c-di-GMP: stimulate in strains with natural and decreased amount but inhibit in the case of high amount of this secondary messenger. P. aeruginosa strains with a normal and elevated level of cyclo-di-GMP appeared to be sensitive to the inhibitory effects of N-benzimidazole-sulfonamide derivatives. The most active derivatives were analogues with nucleophilic substituents. The P. aeruginosa PA01 pJN2133 strain with a low level of the second messenger was not sensitive to the analogs of N-benzimidazole sulfonamide. Bismuth porphyrin complexes are effective inhibitors of the intercellular communication system: they significantly reduce the intensity of the synthesis of autoinducers of the quorum sensing system P. aeruginosa 3-oxo-C12-AHL, C4-AHL, PQS and exoproducts that are under its control: piocyanin, ramolipid and Las-protease. irrespective of the strain-specific level of synthesis of these components. Product Description popup.authors Галкін Борис Миколайович Галкін Микола Борисович Зінченко Оксана Юріївна Русакова Марія Юріївна Семенець Анастасія Сергіївна popup.nrat_date 2020-04-02 Close