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Information × Registration Number 0219U006207, 0118U004369 , R & D reports Title Development of new effective ways of diagnostics and endogenous cytoprotection of ischemic coronary and cerebral blood circulation (clinical and experimental research). popup.stage_title Head Pavlov Serhii Vasylovych, Registration Date 23-04-2019 Organization Zaporozhye State Medical University popup.description2 In vitro research on cell hypoxia modeling has been performed the brain and the heart of the rats. A correlation analysis of synthesis and expression of HSP70 proteins. A study of the nature of expression and synthesis of HSP proteins in conditions of modeling hypoxia in vitro in cells heart with MFP-induced hypoxia in vitro. The concentration of HSP70 in the cells of the heart was determined at hypoxia and against the background introduction of pharmacological agents. The method of PCR has been determined by nature expression of mRNA HSP70 in a suspension of cardiomyocytes under hypoxia and on the background of the introduction of pharmacological agents. The character of expression and the synthesis of HIF proteins and the formation of a thiol-disulphide system under conditions modeling of in vitro hypoxia in heart cells with MFP-induced in vitro hypoxia. The immune enzyme method has been determined by concentration HIF in the cells of the heart with hypoxia and against the background of pharmacological administration agents, and using state of the art biochemical analysis methods thiol-disulphide system of cardiomyocytes (glutathione oxidized, established activity of glutathione reductase, glutathione peroxidase. A comparative analysis of the efficacy of the pharmacological investigations was conducted agents in vitro for their ability to influence the concentration of HSP and HIF proteins and the state of the thiol-disulphide system in the suspension cardiomyocytes. A comparative analysis of the efficacy of the investigators was conducted pharmacological agents in vitro for their ability to influence on the concentration of HSP and HIF proteins and the state of the thiol-disulphide system in suspension of cardiomyocytes. Product Description popup.authors Євсєєва Л.В. Горбачова С.В. Левченко К.В. Нікітченко Ю.В. Робота Д.В. popup.nrat_date 2020-04-02 Close