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0219U100015,
0116U001596
,
Development of basic variants and algorithms using optical biosensors for express control genotoxicity of environmental objects
Starodub Mykola Fedorovych, Доктор біологічних наук
03-01-2019
National University of Life and Environmental Sciences of Ukraine
The object. instrumental analytical tool - biosensor using genetically modified bacterial cells carrying in one operon the referent (reparation system) and indicative (encoding fluorescent proteins) genes for express determination of the level of genotoxicity of a number of chemical environmental factors. The purpose of the work is development of the optimal, simple variants of SOS-type biosenors based on optical fibers capable for expressive determination of the genotoxicity of individual compounds and nanoparticles. Research methods is the detection of genotoxicity of compounds using a biosensor with an integrated SOS system, a DNA damaging agent, and a receptor component with a bioluminescence system. Proposed simple SOS-type biosensor based on optic fiber operating in differential mode, which allows controlling the genotoxicity of environmental objects that are chemicals by its nature. A construction of an optocycler biosensor was carried out to control the genotoxicity of environmental objects which are chemicals by its nature.The developed biosensor has been tested in determining the genotoxicity of referent substances such as: ethanol, dimethyl sulfate and mitomycin C. It is shown that the most accessible and efficient way of functionalizing the surface of the transducer (optrod) is its direct interaction with referent cells located in a cellophane film. Positive results were also obtained by functionalizing the transducer surface with referent cells using sol-gel technology. The genotoxicity of such nanoparticles as AgO, ZnO, CuO, CdO, TiO2 and CeO2 is registered at concentrations starting from 1.0 μg / ml and increased with enlargment of concentrations to 10 μg / ml. We obtained the results which are in good agreement with data shown by other authors for genotoxicity of CuO, AgO, CeO and TiO2 based on the use of several different approaches. The obtained data indicates a significant biological effect of nanoparticles with the size from 50 nm to 100 nm.The
2020-04-02
Updated: 2025-12-19
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