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Information × Registration Number 0219U100042, 0118U003499 , R & D reports Title The search for new strains of nitrogen-fixing microorganisms and replenishment of the collection funds with them popup.stage_title Head Воробей Юлія Олександрівна, Registration Date 09-01-2019 Organization popup.description2  Object of research - the strains of nitrogen-fixing microorganisms. Purpose – to search new strains of diazotrophs and to replenish collection. Research methods: microbiological, biochemical, gasochromatographic and methods of mathematical statistics. As a result of the search for new strains of diazotrophs 12 pure cultures Azospirillum sp. isolated from the rhizosphere of strawberry. It was established that Azospirillum brasilense 68/13 is the most active fixator of molecular nitrogen of the atmosphere (59.15 nmol of ethylene / vessel / day) and is characterized by the highest ability to form biologically active substances. A search and selection of new highly effective strains of Mesorhizobium cicerii was conducted. 69 isolates of bacteria isolated from the nodules of chickpea plants. 31 strains were selected, which according to the morphological and cultural properties corresponded to the characteristics of the genus Mesorhizobium, 14 of them according to physiological and biochemical features are classified as M. siceri. Formation of a significant number of tubers (more than in positive control) was shown after inoculation of seeds by 13 strains from 31 studied isolates As a result of seed inoculation of chickpea seeds Mesorhizobium sp. 101, dry plant mass increased by 13% and dry root weight by 54%. The strain M. siceri 64 contributed to the most significant increase in dry vegetative mass by 39% and dry mass of the roots by 70%. Product Description popup.authors popup.nrat_date 2020-04-02 Close