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Registration Number

0219U100221, 0116U001065 , R & D reports

Title

To Increase the Technology Efficiency of Creation of Spring Barley Doubled Haploid Lines Based on Direct Induction of Embryogenesis in Anther Culture In Vitro

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Head

Bilynska Olena V., Кандидат біологічних наук

Registration Date

31-01-2019

Organization

Plant Production Institute nd. a. V. Ya. Yuriev

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Study Object – embryoid and callus formations, plant regeneration in spring barley anther culture in vitro; barley genotypic diversity for ability to androgenesis in vitro. For the first time, a mechanism of morphogenesis improvement affected by chemically modified starches used as gelling agents instead of agar in spring barley anther culture in vitro was investigated. Particularly, contribution of osmotic and trophic factors was evaluated. New chemically modified starches with improved gelling capacities and ability to promote plant regeneration in spring barley culture in vitro were selected. A patent for invention on “The mode of pretreatment of plant material for spring barley haploid production in anther culture in vitro” was received. When improved technology included such innovative elements as new long-term spike cold pretreatment and replacement of agar with new chemically modified starches was applied to obtain haploids from model genotype, the yield of green plants exceeded 100 % of the total amount of cultivated anthers. A spring barley collection of genotypes related to their diversity for androgenesis in vitro abilities was created. Sources of enhanced capacities for androgenic haploid production were selected. A method for determination of the starch trophic peculiarities based on in vitro cultivation of embryos isolated from barley seeds on media solidified with different starches was developed. A patent application for invention on “The mode of barley immature embryo culture in vitro production” was prepared. The new technical solution makes it possible to avoid time- and labor-consuming procedures of seed sterilization and will accelerate production of aseptic embryo culture. The patentability of the invention on this mode has already been confirmed by UKRPATENT. Scope of Application – investigations in plant cell, tissue and organ in vitro cultivation; breeding aimed at fast generation of starting breeding material in spring barley.

Product Description

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2020-04-02