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Registration Number

0219U100338, 0115U005044 , R & D reports

Title

Production of genetically engineered immunoconjugatesand their application for immunoaffinity chromatography and detection of target antigens

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Head

Horbаtiuk Oksаnа B., Кандидат біологічних наук

Registration Date

07-02-2019

Organization

Institute of Molecular Biology and Genetics of NAS of Ukraine

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A recombinant fusion protein beta-Lac-SPA containing two functional domains - the Staphylococcus aureus protein A, which interacts with the Fc-parts of IgG and bacterial beta-lactamase immune complexes visualization was constructed. Conditions of induction of ?-Lac-SPA synthesis in Escherichia coli, which ensured its accumulation in a soluble form have been determined. The fusion protein ?-Lac-SPA, after isolation and purification, has been tested for the detection of immune complexes in different types of immunoassay and Western Blot. Since Staphylococcus aureus protein A interact with IgG of different species of animals and humans, fusion protein beta-Lac-SPA can be used as a universal secondary immunoassay tools. 

Product Description

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2020-04-02