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Registration Number

0219U100471, 0115U004171 , R & D reports

Title

Studying on plant stress adaptation mechanisms and creation of resistant lines using experimental transgenesis protocos

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Head

Shcherbаk Nаtаliia L., Кандидат біологічних наук

Registration Date

13-02-2019

Organization

Institute of Cell Biology and Genetic Engineering

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  An analysis of genetic constructions with genes contributing to the stress adaptation of plants under the control of the constitutive 35S promoter was carried out. The prospect of their use for obtaining transgenic plants with increased resistance to stress factors was determined. As a result of the experiments, several transgenic potato lines which showed less sensitivity in comparison with control to saline and osmotic stress in vitro were selected. The difference between the control group and resistant transgenic plants was observed both in increasing biomass and in the activity of enzymes of the antioxidant system. The conducted studies confirmed the possibility of obtaining more resistant to saline stress potato plants (Slavianka and Lugovka varieties) bearing a vacuolar antiporter gene HvNHX2 from barley driven by the CaMV 35S promoter. It has also been confirmed that transgenic potato plants with increased proline content are characterized by improved osmotic stress tolerance. The obtained data will allow to continue the project to receive transgenic plants of other species with the abovementioned genes and expand the research of adaptation characteristics of these plants under stress conditions.

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