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Information × Registration Number 0219U100482, 0118U001745 , R & D reports Title Hydrogen-deuterium exchange mass spectrometry as a qualitatively new approach for the investigation of the spatial structure of the eEF1B translation complex in the solution popup.stage_title Head Nehrutskyi Borys S., Доктор біологічних наук Registration Date 14-02-2019 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2  The preparative amount of eEF1Ba, eEF1Bb and eEF1Bg subunits of the complex of elongation translation factors eEF1B was obtained, a high degree of purification and monomeric state of these proteins were confirmed, what is necessary for the further implementation of the project. The procedure of expression and purification of the N-terminal domain of the eEF1Bb subunit was optimized and the recombinant protein was characterized. The hydrogen-deuterium exchange method was used to study the structure and dynamics of the N-terminal domain of the eEF1Bb subunit. For the first time it has been shown that this domain of the eEF1Bb molecule has a dynamic, mobile structure with unstable tertiary contacts. It was concluded that the lability of the structure may be important for the interaction of eEF1Bb with other components of the eEF1B complex, namely, with eEF1Bg and valyl-tRNA synthetase, and may also provide cellular contacts of eEF1Bb associated with the non-translated functions of this protein. Product Description popup.authors popup.nrat_date 2020-04-02 Close