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Information × Registration Number 0219U101134, 0116U000173 , R & D reports Title Development of genotyping and marking systems for valuable biological characteristics in crops popup.stage_title Head Morgun Bogdan V., Кандидат біологічних наук Registration Date 10-05-2019 Organization Institute of Cell Biology and Genetic Engineering popup.description2 To study the genetic diversity of wheat and spelt, 5 different systems based on IRAP have been developed, which allow to effectively detect polymorphic loci between retrotransposons. Transgenic allele extra copies of Glu-A locus have been determined; allele state of Psy1 genes in 165 cultivars and wheat hybrids of different breeding; allelic state of Pina-D1 and Pinb-D1 genes (Pina-D1 gene showed no polymorphism). Complex study of genetic effects of the Gpc-B1 gene, introduced from Triticum turgidum ssp. dicoccoides, has been carried out in the wheat genome. The analysis of F5 wheat plants was conducted: 16 most valuable lines containing the optimal for breadmaking quality formula of Glu-1 locus (Glu-A1a or Glu-A1b, Glu-B1al, Glu-D1d) were selected; 12 unique lines were identified that carried a combination of Pina-D1b, Pinb-D1a alleles, not characteristic of Ukrainian breeds; It was found that due to the gene Gpc-B1 from Triticum turgidum ssp. dicoccoides, the content of total grain protein increased by 14% and the accumulation level of iron, Zn, Mn, Cu, Se and Mg in grains elevated compared to the original cultivar Kuyalnik. It has been established that F6 generation wheat lines (Gpc-B1 gene carriers) are resistant to lodging and have an average yield of 43 c/ha. The chemical mutagenesis of triticale was conducted, which resulted in plants that carry altered genome confirmed by IRAP-markers. The multifactorial dispersion analysis of the effects of various Pinb-D1, Wx-A1, Wx-B1, and Wx-D1 alleles and their combinations on grain hardness of wheat breeding hybrids revealed the combined effect of Wx-A1 and Wx-D1 gene alleles. Full-length SbeIIa and SbeIIb Secale cereale genes were cloned and sequenced; According to the results of exon-intron structure analysis of the SbeII rye genes, it has been established that the SbeIIb gene is characterized by variable long intronic regions (greater than 8 kb), which contain zones with elevated G-C composition. Product Description popup.authors Pokhylko Svitlana Yu. Stepanenko Anton I. Stepanenko Olena V. popup.nrat_date 2020-04-02 Close