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Information × Registration Number 0219U101574, 0119U101611 , R & D reports Title Improvement of capric fatty acid content in Camelina sativa seeds using RNA interference and gene editing technologies. popup.stage_title Head Blume Yaroslav B., Доктор біологічних наук Registration Date 13-09-2019 Organization Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine popup.description2  • T1-seedlings obtained after Camelina sativa transformation using CRISPRCsААЕ15/16 construct were treated by BASTA herbicide in greenhouse. There were no herbicide resistant ones. • Eight transgenic C. sativa lines (Т1 generation) were selected after transformation using RNAiCsААЕ15/16 construct. They were resistant to BASTA treatment and possessed sense-antisense-Pdk-intron-ААЕ15-ААЕ16 sequence as PCR confirmed. Ukrainian FEORZhYaFD line was used as an initial material for transformation. Т1 seed germination, further growth and blooming took place under greenhouse conditions. • Seeds were gathered from transgenic camelina lines which were resistant to BASTA and had foreign chimeric gene. Thus, Т2 generation was obtained. • Increase in capric С10:0 fatty acid content was revealed in oil of Т2 transgenic camelina seeds up to 5% as compared to the controls. Product Description popup.authors Sakhno Liudmyla O. popup.nrat_date 2020-04-02 Close