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Information × Registration Number 0219U102060, 0115U002947 , R & D reports Title Mechanisms of invadopodia functioning and expression of their components in breast cancer popup.stage_title Head Rynditch Alla V., Доктор біологічних наук Registration Date 16-12-2019 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2 The aim is to study the expression, intermolecular interactions and functional characterization of the TKS4 scaffold protein and its isoforms, which are important components of invasive structure, as well as the ITSN2 adapter protein involved in various cellular processes and associated with various pathologies. Results - interaction of TKS4 with 15 partner proteins found: scaffold proteins (ITSN1, ITSN2, TKS5, TKS4); regulators of the actin cytoskelet (N-WASP, CR16); endocytic proteins (OPHN1, AMPH1, BIN1, DNM2, SYNJ1) and cell signaling pathway proteins (CRK, GRB2, PLCγ1 and SRC). A new isoform of TKS4 - TKS4b was cloned and characterized. Based on the obtained data, a model of TKS scaffold protein family involvement in cellular processes was constructed, which testifies to the important role of these proteins in the reorganization of the actin cytoskeleton and the remodeling of the cell membrane - processes that occur during the formation and functioning of invadopodia. Quantitative PCR revealed that the short isoform of intersectin 2 (ITSN2-S) can be used as a prognostic marker for breast cancer.  Product Description popup.authors Gryaznova Tetyana A. Gubernatorova Anastasiya O. Kropyvko Sergii V. Pankivskii Sergii V. Foienko Inna M. popup.nrat_date 2020-04-02 Close