Search academic texts

Information × Registration Number 0219U102124, 0118U002380 , R & D reports Title Structural mechanisms of the tautomerisation of the biologically important H-bonded molecular complexes: the development of the new ideas popup.stage_title Head Brovarets Olha O., Registration Date 19-12-2019 Organization Institute of Molecular Biology and Genetics of NAS of Ukraine popup.description2  It is proved for the first time that biologically important processes of mutagenic tautomerization of G · T (w) ↔G * · T (WC) and A · C (w) ↔A · C * (WC) are carried out through the zwitterionic transition state without the opening of steam and without direct participation endogenous water molecules. The biological importance of tautomerization of pairs of pairs is attributed to the mechanism of formation of enzymatically competent conformations of the DNA base pairs A · C (w) and G · T (w) in the pocket of recognition of high-precision replicative DNA polymerase responsible for the occurrence of spontaneous point inclusion errors. It has been shown for the first time that the modification of uracil (U → Hal5U) lowers the barrier of converting its displaced pairs from G into pairs with the participation of a mutagenic tautomer G having Watson-Crick geometry, thereby causing a mutagenic pressure on DNA. The maximum effect is observed for 5BrU, which is the most effective mutagen among the studied halogen U. Product Description popup.authors Brovarets Olha O. Govorun Dmitro M. popup.nrat_date 2020-04-02 Close