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Information × Registration Number 0219U102219, 0115U005022 , R & D reports Title Selection of drought resistant plant lines using the yeast trehalose biosynthesis genes overexpression popup.stage_title Head Sakhno Liudmyla O., Доктор біологічних наук Registration Date 23-12-2019 Organization Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine popup.description2 Trehalose content has been measured in the leaves of transgenic camelina plants which were obtained using FEORZhYaF-1 line as a background. These trangenics were selected on selective media and the ScTPS2 gene was detected in their DNA as a result of PCR analysis. There was no difference in trehalose content between the control and transgenic plants both under physiological growth conditions and water deficit. The transgenic lines were more tolerant to osmotic stress as compared to the controls but were characterized by lower growth and smaller sizes. The first transgenic generation was obtained after in planta transformation of Triticum aestivum using eight winter varieties of Ukrainian breeding as a background. PCR analysis confirmed transgenic status of selected lines. Preliminary data indicated the tolerance of the lines obtained to water deficit.   Product Description popup.authors Yemets Alla I. Kvasko Anna Yu. Nischenko Larisa V popup.nrat_date 2020-04-02 Close