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Information × Registration Number 0219U102237, 0119U103586 , R & D reports Title Actinomycetes of the rhizosphere Deschampsia antarctica E.Desv. - potential producers of biologically active compounds for medical purposes popup.stage_title Head Gromyko Oleksandr M., Registration Date 23-12-2019 Organization Ivan Franko National University of Lviv popup.description2  Among 43 actinomycetes isolated from the rhizosphere of D. antarctica E.Desv. (Galindez Island, Antarctica) genera Streptomyces, Micromonospora, Umezawaea, Kribbella and Micrococcus were identified. The studied strains are able to delay the growth of a wide range of pathogens: MRSA, multidrug-resistant strains of gram-negative bacteria and fungi. 3 potential producers of anti-TB antibiotics pamamycins and 5 potential producers of cell wall active antibiotics were identified using biosensors. In the genomes of studied actinomycetes biosynthetic genes of PKS I, PKS II and NRPS were detected using PCR-screening. The 26 most promising actinomycetes cultures were selected to evaluate their chemical structure of the synthesized BACs. The most optimal nutrient media for BACs synthesis are determined by: solid SG and ISP2; liquid Um1, DNPM and NL19. HPLC-MS analysis of secondary metabolites of isolate Umezawaea sp. Da 62-2, which grew in different production media, identified antimalarial compounds phloeodictyne C1 and C2. These compounds were isolated only from the haploscleridas sponges. Potentially new two compounds of mass m/z 242,2480 [M+H]+ and m/z 398,3420 [M+H]+ were also found. The strain of Umezawaea sp. Da 62-2 was deposited in the State depositary of Danylo Zabolotny Institute of Microbiology and Virology NAS of Ukraine. Product Description popup.authors Bilyk Bohdan R. Leshchynska Oksana M. Mytsyk Julia Y. Syrvatka Vasyl J. Fedorenko Viktor O. popup.nrat_date 2020-04-02 Close