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Information × Registration Number 0219U102268, 0119U002995 , R & D reports Title Obtaining and molecular-biochemical characterization of fibrin (gene) lytic enzymes from Antarctic marine hydrobionts. popup.stage_title Head Savchuk Olexiy M., Доктор біологічних наук Registration Date 26-12-2019 Organization Taras Shevchenko National University of Kyiv popup.description2  During the research, the conditions for the receipt of functionally active fibrinogenolytic enzymes from the tissues of the Antarctic region's hydrobionts were optimized. The enzyme preparation scheme included several chromatographic steps, in particular, affinity chromatography on a Blue Sepharose column and size separating chromatography on a Superdex 200 column. As a result of this methodological approach from S. neumayeri, O. validus and P. tissues. corrugatus obtained fibrinogenolytic enzymes with a molecular weight of 34 kDa, 28 kDa and 26 kDa, respectively. The ability of fibrinogenolytic enzymes from the tissues of S. neumayeri and O. validus to cleave primarily the A? Chain of a fibrinogen molecule indirectly indicates their belonging to? Fibrinogenases. Prolonged incubation of enzymes with fibrinogen was accompanied by cleavage of the β-chain as well. Enzymes from P. corrugatus tissues were cleaved by fibrinogen only under long-term incubation. The fibrinogenolytic enzymes obtained from the tissues of all the aquatic organisms studied caused impaired ability of the fibrinogen molecules to polymerize and form a fibrin clot, which was confirmed by the results of basic chronometric tests, in particular, "Activated partially by thrombin time", "Prothrombin time", "Prothrombin time". Inhibition of the process of platelet aggregation was detected by incubation of the obtained fibrinogenolytic enzymes with platelet-enriched plasma. According to the results of the substrate analysis, fibrinogenolytic enzymes showed higher activity against substrates containing the Arg residue in the P1 position, compared to the substrate containing the Lys residue in this position. Product Description popup.authors Vovk Tatiana B. Galenova Tetyana I. Raksha Nataliya G. Savchuk Olexiy M. popup.nrat_date 2020-04-02 Close