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Information × Registration Number 0220U000152, 0115U003617 , R & D reports Title Cellular and molecular mechanisms of regulation of photosynthesis in biotechnological plants resistant to abiotic stresses popup.stage_title Head Stasik Oleg, д.б.н. Registration Date 20-01-2020 Organization Institute of Plant Physiology and Genetics NASU popup.description2 The features of the photosynthetic apparatus reaction to soil drought and the level of polymorphism of DNA patch, flanked by inverted LTR repetitions of retrotransposons were studied in genetically modified wheat plants of variety Zimoyarka obtained by the method of Agrobacterium-mediated transformation using the pBi-OAT vector with the target gene, ornithine-?-aminotransferase of Medicago truncatula and the selective gene of neomycin phosphotransferase II (nptII) E. coli, the binary vector construct pBi2E, which contains a heterologous double-stranded RNA suppessor of the proline dehydrogenase ProDH of Arabidopsis and a selective gene neomycin phosphotransferase II (nptII) E. coli, or only a selective gene neomycin phosphotransferase II (nptII) E. coli. It was found that genetically modified wheat plants with reduced gene expression of proline dehydrogenase have an increased level of proline and improved drought tolerance of the photosynthetic apparatus associated with an increase in the activity of antioxidant chloroplast enzymes. When using highly efficient primers for retrotransposons Sukkula, Sabrina, Wham, Nikita and Wilma1, DNA polymorphism was not found in these plants. In genetically modified wheat plants containing the ornithine-?-aminotransferase gene and/or the selective neomycin phosphotransferase II gene, an increase in the level of DNA polymorphism was revealed, however, there were no significant changes in the characteristics of the photosynthetic apparatus under optimal irrigation conditions or in conditions of soil drought. The results obtained indicate that an improvement in the adaptive capabilities of the photosynthetic apparatus in plants that contain a suppressor of the proline dehydrogenase gene is associated with an increase in proline levels and an increase in the efficiency of regulatory and protective systems. Product Description popup.authors Дубровна Оксана Василівна Зборівська Оксана Валеріївна Кірізій Дмитро Анатолійович Осипова Оксана Леонідівна Соколовська-Сергієнко Оксана Григорівна Троценко Віта Анатоліївна popup.nrat_date 2020-04-02 Close