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Information × Registration Number 0220U001165, 0117U002427 , R & D reports Title The role of signal mediators and compounds with hormonal activity in the formation of adaptive plant responses to abiotic stressors popup.stage_title Head Kolupaev Yuriy Evgenovych, Доктор біологічних наук Registration Date 30-01-2020 Organization V.V. Dokuchaev Kharkiv National Agrarian University popup.description2 Object of study - processes of functional interaction of signaling intermediates namely hydrogen sulfide, nitric oxide, reactive oxygen species (ROS) and stress phytohormones when inducing plant resistance to adverse abiotic factors. Purpose of the work - to establish the role of hydrogen sulfide and its functional interaction with ROS in the implementation of stress-protective effect of exogenous salicylic acid on plants; to assess the possible value of salicylate and jasmonate signaling in the implementation of the hydrogen sulfide physiological effects; to prove the possibility of enhancing the stress-protective effects of phytohormones when used in combination with donors of signaling molecules - hydrogen sulfide and nitric oxide; to create theoretical foundations for using a hydrogen sulfide donor sodium hydrosulfide as a physiologically active substance in crop production. Research methods - biochemical, physiological, field, statistical. By studying the dynamics of H2S content and using inhibitory analysis, the participation of hydrogen sulfide as a signaling mediator in the process of inducing heat resistance in wheat seedlings by the action of exogenous salicylic acid is proved, and also it's functional relationship with hydrogen peroxide. The enhancement of the stress-protective effect of salicylic acid on wheat seedlings at hyperthermia by the NaHS hydrogen sulfide donor was shown. Using Arabidopsis mutants defective in jasmonate signaling (jin1 and coi1), its significance for the realization of the protective effects of exogenous hydrogen sulfide and nitric oxide under salt stress has been established. At the same time, it was shown that the effects of inducing salt resistance in salicylate-deficient Arabidopsis plants (NahG transformants) by the NaHS action did not differ much from those in wild-type plants. This indicates that salicylic acid does not participate in transduction of the H2S signal in inducing plants' salt tolerance. It has been shown that pre-sowing treatment of winter wheat and rye seeds with solutions of a nitric oxide donor sodium nitroprusside (SNP) enhances the effect of cold hardening of seedlings, increasing their frost resistance. This phenomenon was accompanied by an increase in the content of sugars, proline, anthocyanins, and flavonoids in seedlings, as well as the activity of superoxide dismutase and guaiacol peroxidase. In the combined pre-sowing treatment with solutions of SNP and salicylic acid, their cryoprotective effects on cereal seedlings were enhanced. A synergy of stress-protective effect of the brassinosteroid 24-epibrasinolide (24-EBL) and the donor of nitric oxide SNP in low concentrations on the heat resistance of wheat seedlings was established. A significant increase in the activity of superoxide dismutase and a decrease in oxidative damage to plants under the influence of the combination of 24-EBL and SNP were noted. It has been proven that treatment with hydrogen sulfide donor NaHS reduces the negative effects of natural drought on the pigment pool, water metabolism and productivity of spring wheat. In general, the results obtained are the theoretical basis for techniques for increasing the heat, drought, and salt resistance of plants. Product Description popup.authors Гавва (Фірсова) Катерина Миколаївна Горєлова Олена Іванівна Заярна Олена Юріївна Карпець Юрій Вікторович Кокорев Олександр Ігоревич Лугова Ганна Арнольдівна Швиденко Микола Володимирович Шкляревський Максим Анатолійович Ястреб Тетяна Олегівна popup.nrat_date 2020-04-02 Close